Pharmacological study of the possible therapeutic effect of certain natural and synthetic drugs in experimentally induced osteoporosis in rat /
دراسة فارماكولوجية للتاثير الدوائى المحتمل لبعض المواد الطبيعية و المشيدة فى هشاشة العظام المحدثة معمليا فى الجرذان
Mohamed Abdelaziz Mahmoud Ibrahim ; Supervised Azza M. Agha , Osama Elshabrawy
- Cairo : Mohamed Abdelaziz Mahmoud Ibrahim , 2014
- 120 P. : charts , facsimiles ; 25cm
Thesis (M.Sc.) - Cairo University - Faculty of Pharmacy - Department of Pharmacology and Toxicology
The current study investigated the antiosteoporotic effects of linseed and fish oils as natural sources of polyunsaturated fatty acids on ovariectomy-induced osteoporosis as compared to alendronate sodium; a nitrogen containing bisphosphonate in this model of experimental osteoporosis. Rats were rendered osteoporotic through bilateral ovariectomy and kept on AIN-93 modified diet for 20 weeks. Linseed oil (90 and 180 mg/kg, p.o), fish oil (90 and180 mg/kg, p.o), and alendronate sodium (3 mg/kg, p.o) were administered to rats for 8 weeks. Efficacy of tested agents was judged based on the estimation of serum calcium (Ca+2), phosphorus, osteocalcin (OCN), bone alkaline phosphatase (BALP), tartrate resistant acid phosphatase (TRAP), tumor necrosis factor alpha (TNF-Ü), as well as histopathological examination of bone sections. Besides, bone mineral densities (BMD) of femur, tibia, and spine were measured by dual x-ray absorptiometry to evaluate the effect of the tested drugs on calcium deposition in bone. Linseed and fish oils hampered serum levels of Ca+2, OCN, BALP, TRAP, and TNF-Ü after both 4 and 8 weeks of its administration to the osteoporotic rats along with an increment in serum phosphorus and femur, spine, and tibia BMD as compared to alendronate sodium. Linseed oil and to a better extent fish oil possess promising anti-osteoporotic effects comparable to alendronate that might be attributed to their contents of polyunsaturated fatty acids. Hence, it can be recommended to use them in osteoporotic patients