TY  - BOOK
AU  - Hanaa Mohamed Mohamed Elzahed 
AU  - Amany Fahmy Hanon , 
AU  - Dina SabryAbdelfatah , 
AU  - Osama Mohamed Ebrahim Albarany , 
TI  - Molecular identification of blood stains on different surfaces and fabrics using specific multiplex mRNA  / 
PY  - 2014///
CY  - Cairo : 
PB  - Hanaa Mohamed Mohamed Elzahed , 
KW  - Blood stains
KW  - Body fluid identification
KW  - globin genes
N1  - Thesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Forensic Medicine and Clinical Toxicology; Issued also as CD
N2  -  In current forensic practice, blood stain identification is determined using  enzymatic, immunologic and chemical testing. Recently, mRNA-profiling has emerged as an alternative strategy to identify the blood stains. mRNA profile clearly reveals tissue-specific gene expression patterns. HBB and HBA (the alpha and beta subunits of hemoglobin A) are specific mRNA blood biomarkers.  This study aimed  at identification of  blood stains on different tested surfaces and fabrics for forensic application by molecular assessment of specific mRNA blood biomarkers. For this study, 100 blood samples were collected from  healthy volunteers and they were classified  into: (1) 9 groups, each group included 10 samples that were applied on 9 different fabrics and surfaces; cotton, wool, silk, synthetic nylon, wood, glass, ceramic, iron as a metal and leather. (2) One blood group  included 10 samples that were applied on glass and  exposed to humidity and high temperature to test their effect on mRNA. Ten saliva samples were collected from  healthy volunteers  and were applied on glass as a negative control body fluid. All samples were left to dry for 1 day. The next steps  of  the study  were  RNA extraction from blood and saliva dried stains, reverse  transcription, performing gene expression analysis of the  blood specific markers  using RT-PCR  then detection of the amplified gene using  agarose gel electrophoresis. The results demonstrated that  HBB and HBA can be recovered from blood stains in sufficient quantity and quality for mRNA  analysis
ER  -