Ahmed Ibrahim Abdelfattah Nossier <br/><br/>
Diagnosis of bladder cancer using nanotechnology /  
 تشخيص سرطان المثانة باستخدام تكنولوجيا النانو 
Ahmed Ibrahim Abdelfattah Nossier ; Supervised Mohamed Ahmed Hamdy , Manal Fouad Ismail , Sanaa Eissa Mohamed 
 - Cairo :   Ahmed Ibrahim Abdelfattah Nossier ,   2014 
 - 229 P. :   charts , facsimiles ;   25cm 
<br/><br/>Thesis (Ph.D.) - Cairo University - Faculty of Pharmacy - Department of Biochemistry 
<br/><br/> Hyaluronidase (HAase) was reported as a urinary marker of bladder cancer. In this study, a simple colorimetric gold nanoparticle (AuNP) assay was developed for rapid and sensitive detection of urinary HAase activity. Charge interaction between polyanionic hyaluronic acid (HA) and cationic AuNPs stabilized with cetyl trimethyl ammonium bromide (CTAB) led to formation of gold aggregates and a red to blue color shift. HAase digests HA into small fragments preventing the aggregation of cationic AuNPs. The nonspecific aggregation of AuNPs in urine samples was overcome by pre-treatment of samples with the polycationic chitosan that was able to agglomerate all negatively charged interfering moieties before performing the assay  
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<br/><br/><label>Subjects--Index Terms: </label>Bladder cancer Hyaluronic acid Hyaluronidase