TY  - BOOK
AU  - Ahmed Ibrahim Abdelfattah Nossier
AU  - Manal Fouad Ismail  , 
AU  - Mohamed Ahmed Hamdy , 
AU  - Sanaa Eissa Mohamed , 
TI  - Diagnosis of bladder cancer using nanotechnology / 
PY  - 2014///
CY  - Cairo : 
PB  - Ahmed Ibrahim Abdelfattah Nossier , 
KW  - Bladder cancer
KW  - Hyaluronic acid
KW  - Hyaluronidase
N1  - Thesis (Ph.D.) - Cairo University - Faculty of Pharmacy - Department of Biochemistry; Issued also as CD
N2  - Hyaluronidase (HAase) was reported as a urinary marker of bladder cancer. In this study, a simple colorimetric gold nanoparticle (AuNP) assay was developed for rapid and sensitive detection of urinary HAase activity. Charge interaction between polyanionic hyaluronic acid (HA) and cationic AuNPs stabilized with cetyl trimethyl ammonium bromide (CTAB) led to formation of gold aggregates and a red to blue color shift. HAase digests HA into small fragments preventing the aggregation of cationic AuNPs. The nonspecific aggregation of AuNPs in urine samples was overcome by pre-treatment of samples with the polycationic chitosan that was able to agglomerate all negatively charged interfering moieties before performing the assay
UR  - http://172.23.153.220/th.pdf
ER  -