Mohamed Gamal Salah Farahat  <br/><br/>
Expression and purification of recombinant histidine-tagged L-asparaginase II from escherichia coli /  
 معاد الاتحاد المعلم بالهيستيدين من الايشيريشيا كولاى IIتعبير وتنقية ل-اسبراجيناز 
Mohamed Gamal Salah Farahat ; Supervised Zienat Kamel Mohamed Ibrahim , AlaaEddeen Seufi Mohamed , Sherif Mohamed Elnagdy  
 - Cairo :   Mohamed Gamal Salah Farahat  ,   2014 
 - 178 P. :   charts , facsimiles ;   25cm 
<br/><br/>Thesis (Ph.D.) - Cairo University - Faculty of Science - Department of Botany Microbiology 
<br/><br/> L-asparaginase (L-ASNase) has been widely used as a therapeutic agent in the treatment of various lymphoblastic leukemia diseases. In this study, Escherichia coli MG27 was isolated from the River Nile and subjected to optimization studies to maximize the production of L-ASNase. ansB gene, encoding L-ASNase II from E. coli MG27, was cloned into pQE-30 expression vector and expressed in E. coli M15. The recombinant histidine-tagged L-ASNase II was purified to homogeneity by NiNTA affinity chromatography and displayed a single 36.0 kDa band on SDS-PAGE 
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<br/><br/><label>Subjects--Index Terms: </label>L-Asparaginase Optimization Screening