TY  - BOOK
AU  - Mohamed Gamal Salah Farahat 
AU  - Alaa Eddeen Seufi Mohamed , 
AU  - Sherif Mohamed Elnagdy  , 
AU  - Zienat Kamel Mohamed Ibrahim  , 
TI  - Expression and purification of recombinant histidine-tagged L-asparaginase II from escherichia coli / 
PY  - 2014///
CY  - Cairo : 
PB  - Mohamed Gamal Salah Farahat  , 
KW  - L-Asparaginase
KW  - Optimization
KW  - Screening
N1  - Thesis (Ph.D.) - Cairo University - Faculty of Science - Department of Botany Microbiology; Issued also as CD
N2  - L-asparaginase (L-ASNase) has been widely used as a therapeutic agent in the treatment of various lymphoblastic leukemia diseases. In this study, Escherichia coli MG27 was isolated from the River Nile and subjected to optimization studies to maximize the production of L-ASNase. ansB gene, encoding L-ASNase II from E. coli MG27, was cloned into pQE-30 expression vector and expressed in E. coli M15. The recombinant histidine-tagged L-ASNase II was purified to homogeneity by Ni{u2013}NTA affinity chromatography and displayed a single 36.0 kDa band on SDS-PAGE
ER  -