Enhancement of azo dyes biodegradation by bioaugmentation and immobilization of microbial cells in wastewaters /
تسريع التكسير الحيوي لصبغات الأزو بواسطة زيادة المعالجة الحيوية والتشبع الوضعي للخلايا الميكروبية في مياه الصرف الصحي
Walaa Ahmed Ameen Alshareef ; Supervised Mohammad A. Ramadan , Abdelgawad M. Hashem , Tamer Essam
- Cairo : Walaa Ahmed Ameen Alshareef , 2014
- 165 P. : charts ; 25cm
Thesis (Ph.D.) - Cairo University - Faculty of Pharmacy - Department of Microbiology and Immunology
Biological decolorization of dyeeffluent is receiving much consideration due to cost effective and its safety use. A total of 35 isolates (8 bacteria and 17 fungi) were recovered from waste waters by the enrichment culture technique, using Reactive black 5 (RB5) as a sole carbon source. A bacterial isolate (identified as Micrococcus luteus) showed the highest biodegradation activity. This isolate was able to remove up to 66% of the dye within 3 days of incubation. Comparatively, 53 % of the dye was decolourized by a yeast isolate (identified as Candida albicans) after 3 days of incubation. Addition of yeast extract at a concentration of 10 g l-1 enhanced the biodegradation, where complete disappearance of the dye was observed within 3 days of incubation. Interestingly, the bacterium was able to decolorize the dye up to a concentration of 40 mg l-1. The residual of the dye after degradation, was traced, where a metabolite was detected by TLC, UV absorbance as well as HPLC after 5 days of incubation by Micrococcus luteus. The metabolite was detected by TLC with Rf= 0.8 and a wavelength of 597 nm