Marwa Nageh Abdelraouf Mahmoud <br/><br/>
Evaluation of the genetic polymorphisms rate of the tumor  necrosis factor- alpha gene at  positions -238 and -308 in the Egyptian  hepatitis c virus patients /  
 تقييم معدل تعدد الاشكال المظهرية لجين عامل النخر الورمى الفا فى الموضعين -832 و -302 فى المرضى المصريين المصابين بالفيروس الكبدى الوبائى سى  
Marwa Nageh Abdelraouf Mahmoud ; Supervised M. Akmal Abdelraheim Elghor , Magda Mohmmed Noshy , Hanaa Ahmed Amer  
 - Cairo :   Marwa Nageh Abdelraouf Mahmoud ,   2014 
 - 118 P. :   charts , facsimiles ;   25cm 
<br/><br/>Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Zoology   
<br/><br/> Tumor necrosis factor-alpha (TNF-Ü) is an important cytokine in  generating an immune response against infection with hepatitis C virus  (HCV). In HCV infection, the production of inappropriate cytokine levels  may contribute to viral persistence. The G  A transition at position -308  and -238 in the TNF-Ü promoter has shown to affect the overall expression and the secretion of TNF-Ü. The aim of this study was to investigate the  prevalence of genotypes associated with polymorphisms of TNF-Ü  promoter at position -308 and -238 in a group of Egyptian HCV- infected  patients compared with healthy uninfected control subjects. In addition,  the association between these polymorphisms and the clinical  characteristics of the positive HCV patients were investigated. Genotyping was determined by polymerase chain reaction (PCR) followed by restriction fragment length polymorphism analysis (RFLP) on genomic  DNA isolated from 61 HCV patients and 20 healthy uninfected control  
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<br/><br/><label>Subjects--Index Terms: </label>Hepatitis C virus,    Polymerase chain reaction Tumor necrosis factors- alpha