Mai Ibrahim Mahmoud Ibrahim

Correlation between antibiotic resistant Salmonella in chicken, antibiotic residues and antibiotic resistance genes / اعلاقة اسالمو المقاومة مضادات احيو فى اد ج و بقا المضادات الحيوية والجينات المقاومة لهذه المضادات Mai Ibrahim Mahmoud Ibrahim ; Supervised Salah Eldin Abedelkreem Selim , Mahmoud Dardiri Elhariri - Cairo : Mai Ibrahim Mahmoud Ibrahim , 2014 - 131 P. : charts , facsimiles ; 25cm

Thesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology

Salmonella is considered the most important bacterial diseases that infect poultry and transmitted to human. Isolation of Salmonella from tissues of poultry needs firstly inoculation of the tissues in enrichment media that inhibit growth of bacteria other than Salmonella and helps in propagation of Salmonella. By testing several enrichment media as RV, TT and SF, it was found that RV is the best one. Serotyping of Salmonella isolates is the most important steps necessary for Salmonella identification, but because of the numerous difficulties in this method, the use of molecular biology techniques in achieving this goal is very imprtant. PCR and sequencing of inv A gene were used for Salmonella identification. Through molecular biology and informatics and comparison with data on Gene- Bank, 36 isolates of Salmonella were identified to 8 S. Entritidis, 18 S. Heidelberg, 6 S. Gallinarum and 1 S. Newport, there were 3 isolates untyped. Serological test for 12 isolates have been selected randomly, the results revealed that, 9 S



Chicken PCR and four Salmonella