Rana Mohamed Ibrahim <br/><br/>
Pharmacognostical study of securigera securidaca (Linnaeus) degen and doerfler :   Family fabaceae /  
 دراسة عقاقيرية علي نبات حلبة الخيل (لينيه) ديجين و دورفيلر - العائلة البقلية  
Rana Mohamed Ibrahim ; Supervised Seham Salah Eldin Elhawary , Abdelrahman Omar Elshabrawy , Ali Mahmoud Elhalawany 
 - Cairo :   Rana Mohamed Ibrahim ,   2014 
 - 400 P. :   charts , facsimiles ;   25cm 
<br/><br/>Thesis (M.Sc.) - Cairo University - Faculty of Pharmacy - Department of Pharmacognosy 
<br/><br/> DNA and botanical profiling of Securigera securidaca L. were conducted. The lipoidal content of the flowers was investigated using GC/Ms analysis. Two compounds were isolated from n-hexane fraction of the flowers and identified as Ý-sitosterol and Ü-amyrin. One phenolic acid; salicylic acid was  isolated from the methylene chloride fraction. Three flavonoids were isolated  from the ethyl acetate fraction and identified as isovitexin, isoquercetrin and  isorhamnetin-3-O-Ý-D-glucoside. Total flavonoids and phenolics were  determined colorimetrically. The amount of total flavonoids was 48.82 ± 1.95  mg and total phenolics was 82.39 ± 2.79 mg per g of the dried powdered flowers. HPLC analysis of phenolic compounds present in the flowers revealed that isoquercetrin was the major flavonoid and syringic acid was the  major phenolic acid. HPLC-DAD/ESI-MS analysis of the ethanolic extract of  the flowers allowed the identification of 47 compounds including 39 flavonoids and 8 phenolic acids. One cardenolide was isolated from the  immature fruits and identified as hyrcanoside 
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<br/><br/><label>Subjects--Index Terms: </label>GC/MS  Llipoidal contents Securigera securidaca