TY  - BOOK
AU  - Alia Ahmed Amer
AU  - Ayman Yehia Amin , 
AU  - Mohamed Ramadan Abuelella Nesiem , 
AU  - Shadia Kotb Ahmed , 
TI  - Physiological studies to enhance the production of secondary metabolites using agrobacterium rhizogenes and in vitro hairy root formation on some medicinal plants / 
PY  - 2014///
CY  - Cairo : 
PB  - Alia Ahmed Amer , 
KW  - Agrobacterium rhizogenes
KW  - Hairy root culture
KW  - Hyoscyamus muticus
N1  - Thesis (Ph.D.) - Cairo University - Faculty of Agriculture - Department of Agricultural Botany; Issued also as CD
N2  - This investigation was carried out during the period from 2010 until 2014 in  Central Lab of Plant Physiology, Faculty Agriculture Cairo University Giza, Egypt.  This work was conducted to find suitable protocol to increase the productivity of  Hyoscyamus muticus active ingredients (hyoscyamine and scopolamine) through  hairy roots technique using gene transformation with different strains of  Agrobacterium rhizogenes. Optimization of callus culture conditions from leaf  explants of H. muticus as well as comparing the alkaloids (hyoscyamine and  scopolamine) concentrations from different sources, i.e. three clones of hairy root  cultures, callus cultures and intact normal H. muticus plants
ER  -