TY  - BOOK
AU  - Hala Ahmed Abdelsamie
AU  - Azza Ahmed Aboulenein , 
AU  - Eman Abdel Mohsen Shaheen , 
AU  - Mona Mohamed Hamdy Mahmoud , 
TI  - Detection of hepcidin M-RNA expression in B-thalassemia patient / 
PY  - 2014///
CY  - Cairo : 
PB  - Hala Ahmed Abdelsamie , 
KW  - Hepcidin
KW  - Real-Time PCR
KW  - SYBR Green
N1  - Thesis (M.Sc.) - Cairo University - Faculty of Medicine - Department of Clinical and Chemical Pathology; Issued also as CD
N2  - Background and Objectives: Iron{u2013}Loading anemias are characterized by ineffective erythropoiesis and increased intestinal iron absorption. Erythrocyte transfusions further exacerbate the iron overload. The development of hepcidin- based diagnostics and therapies for iron loading anemias may offer more effective approaches to prevent the toxicity associated with iron overload. The most common iron- loading anemias are major forms of Ý-thalassemia. This study aimed to evaluate the expression of hepcidin mRNA in Ý-thalassemia major patients. Methods: mRNA from a total of 50 Ý-thalassemia major patients and 20 healthy control subjects was extracted, converted to complementary DNA (cDNA) using reverse transcriptase enzyme and then Quantitative detection of hepcidin transcript using ABI Prism (7700) SYBR Green (Real Time-PCR) was done
UR  - http://172.23.153.220/th.pdf
ER  -