TY - BOOK AU - Radwa Wagih Abdelbaset Mahmoud AU - Dina Mohamed Hassan , AU - Heba Taher Othman , AU - Walaa ElSharany Abuelhamd , TI - Role of respiratory syncytial virus and human metapneumovirus in severe lower respiratory tract infection in under 2years old children admitted to intensive care unit at pediatric cairo university hospital / PY - 2015/// CY - Cairo : PB - Radwa Wagih Abdelbaset Mahmoud , KW - Human metapneumovirus KW - Respiratory syncytial virus KW - Severe lower respiratory N1 - Thesis (M.Sc.) - Cairo University - Faculty of Medicine - Department of Pediatrics; Issued also as CD N2 - Background: Viruses are the most important causative agents of acute lower respiratory tract infections (ALRI), ranked as the second leading cause of death and the primary cause of hospitalisation in children. Respiratory syncytial virus (RSV) and human metapneumovirus (hMPV) are among the commonest viral causes of severe ALRTI. Aim: To study the burden of both RSV and hMPV in causing severe ALRI in children less than 2years admitted to PICU at a tertiary care hospital. Setting and Design: Five months prospective observational study in a pediatric hospital of Cairo University. Material and Methods: Nasopharyngeal swabs were collected from children admitted into PICU with diagnosis of community-acquired ALRI who were aged 0-2 years. Samples were transported to the laboratory at (-80{u00B0}C) in viral transport media and processed for detection of RSV and hMPV using real-time polymerase chain reaction (RT-PCR) ER -