TY  - BOOK
AU  - Noha Talal Abdelhalim 
AU  - Abdelhakim Saad  , 
AU  - Hani Mohamed Hassan , 
AU  - Kohar Garo Varjabedian , 
TI  - Immunolog1cal and histopathological detection of digenetic infections in different species of Egyptian fresh water snails / 
PY  - 2015///
CY  - Cairo : 
PB  - Noha Talal Abdelhalim , 
KW  - DNA
KW  - Melanoides tuberculata
KW  - PCR
N1  - Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Zoology; Issued also as CD
N2  - Many snails act as intermediate hosts of medically and veterinary important trematodesthat infect  humans or life stock animals.Four types of cercariae were found, Xiphidiocercarie, Furcocercous, Pleurolophocercous and Gymnocephalous among the four species of snails (Melanoidestuberculata, Bulinustruncatus, Biomphalariaalexandrina and Lymnaeanatalensis) .The data on DNA strand  breakage, expressed as the mean tail DNA content and the mean tail length in hemocytes of M. tuberculata, L.natalensis, B. truncatus and B. alexandrina exposed to parasite infection was  studied.Considering the four different classes of DNA damage, a highly significant difference among  different snail groups was observed. A strong dose{u2013} response relationship was observed for the classes  0 and 3.Snails were individually collected, assayed by the PCR, and showed negative, while DNA extract from individual infected snails was examined different number of bands of amplification ladder  bands seems to be related to the tested parasite concentration.Identification of infected snails when its DNA is pooled with DNA aliquots from several uninfected snails should increase the feasibility of using PCR assay for mass screening of infection. This is expected to be possible only when the  detection sensitivity is very high
UR  - http://172.23.153.220/th.pdf
ER  -