TY  - BOOK
AU  - Marwa Mohamed Mohamed Mohamed Abdalla 
AU  - Fathy Abdalgalel , 
AU  - Iman M. kamel Abumourad , 
TI  - Biochemical and molecular studies on the immune system in association with a zoonotic bacterial infection in aquaculture / 
PY  - 2014///
CY  - Cairo : 
PB  - Marwa Mohamed Mohamed Mohamed Abdalla , 
KW  - Alpha 2-Macroglobulin
KW  - Food- borne disease
KW  - Zoonotic Disease
N1  - Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Biochemistry; Issued also as CD
N2  - The present study presents the expression of A2M gene in the O.  niloticus and albino rats as an immune response to their infection with Zoonotic  bacteria. The infection established in fish with IP injection of 0.3 ml of 3X108  CFU{u2215}ml of C. perfringens, and in rats by feeding on insufficient cooked infected  O. niloticus, Biochemical and molecular studies were established after 3- and 6- days post challenge. Liver functions (ALT, AST and ALP) were significantly  (P{u2264}0.05) highly elevated. Kidney functions (Urea and uric acid) were not  affected but the Cr. showed significant increase in its level (P{u2264}0.05). And the  Ca. showed significant decrease in its level (P{u2264}0.05). CRP elevated in rats and  O. niloticus. Expression pattern of A2M gene was investigated using a semi- quantitative RT-PCR in liver, spleen and kidney tissues, which showed high  expression after the bacterial infection indicating that A2M is immune responder  against C. perfringens infection
UR  - http://172.23.153.220/th.pdf
ER  -