Ahmed Youssef Hassan Ahmed <br/><br/>
Diagnosis of bacterial peritonitis by identification of 16s rRNA genes VIA polymerase chain reaction (PCR) /  
  بواسطة تفاعل البلمرة المتسلسلs16 rRNA تشخيص الالتهاب البكتيرى البريتونى العفوى بالكشف عن جينات       
Ahmed Youssef Hassan Ahmed ; Supervised Mona Ahmed Amin , Ahmed Ibrahim Abdelghani Elbadry , Dina Sabry Abdelfattah Hamed 
 - Cairo :   Ahmed Youssef Hassan Ahmed ,   2015 
 - 122 P. :   charts , facsimiles ;   25cm 
<br/><br/>Thesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Internal Medicine 
<br/><br/> Objectives: evaluate the value of ascitic bacterial 16S rRNA gene determination in the rapid diagnosis of SBP. Results: 16s rRNA gene expression in ascitic fluid was positive in all 100  patients suspected to have SBP even in CNNNA, culture negative or patients had PMN < 250 cells/mm3. There was no correlation between 16s  rRNA levels and the different laboratory parameters of our cases, it is only  correlated with the results of ascitic fluid culture where its levels was  higher in culture positive compared to those with culture negative. Conclusion: Identification of bacterial 16s rRNA in ascitic fluid has its  significant clinical and diagnostic relevance in suspicion of SBP and its  prompt therapy especially in CNNNA. It might be an alternative diagnostic tool to ascitic fluid bacterial culture and PMN in early diagnosis and prompt  treatment with subsequent improvement of survival which needs further investigations  
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<br/><br/><label>Subjects--Index Terms: </label>16s rRNA gene  Liver cirrhosis    Spontaneous bacterial peritonitis (SBP)