TY  - BOOK
AU  - Heba Allah Elsayed Ibrahim Mohammed Seyam
AU  - Emtithal Mohammed Abdelsamiea , 
AU  - Fatma Kamel Adham , 
AU  - Sawsan Elmohandes , 
TI  - First detection of deformed wing virus (DWV) and Kakugo virus (KV) in honeybees (Apis mellifera L.) (Hymenoptera - Apidae) in Egypt by RT - PCR / 
PY  - 2015///
CY  - Cairo : 
PB  - Heba Allah Elsayed Ibrahim Mohammed Seyam , 
KW  - Deformed wing virus
KW  - Kakugo virus
KW  - Varroa mites and RT - PCR
N1  - Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Entomology; Issued also as CD
N2  - Total RNA was isolated for deformed wing virus (DWV) and Kakugo virus (KV) from honeybees. Viral detection and identification was confirmed by sequencing of the PCR  products using conventional RT - PCR. Homology search of DWV PCR amplified fragment revealed similarities of 99% with DWV isolate Warwick - 2009 poly - protein gene (accession number: GU109335.1) and 98% with DWV isolate Chilensis A complete genome (accession number: JQ413340.1). Also, homology search of KV PCR amplified  fragment revealed similarities of 97% with KV isolate - Ox genomic sequence (accession  number: KC786224.1), 90.9% with DWV - Ox genomic sequence (accession number: KC786223.1) The described real - time RT - PCR SG RT - PCR proved to be a fast, accurate and useful technique to detect and quantify low viral loads in honeybees and varroa mites
ER  -