TY  - BOOK
AU  - Asem Metwally Aboshanab Salman 
AU  - Abdelgawad Ali Fahmi , 
AU  - Mohamed Ali Eldosouky , 
AU  - Naglaa Mohamed Kholouss , 
TI  - Evaluation of glutathione S-transferase M1 genotype and neopterin as prognostic factors in  Ý-thalassemia patients / 
PY  - 2015///
CY  - Cairo : 
PB  - Asem Metwally Aboshanab Salman  , 
KW  - Cytokines
KW  - GSTM1
KW  - Neopterin
N1  - Thesis (Ph.D.) - Cairo University - Faculty of Science - Department of Biochemistry; Issued also as CD
N2  - This work was carried out to investigate the role of Neopterin and GSTM1  polymorphism in prognostic Ý-thalassemia, also to detect the correlation between  GSTM1 null genotype and appearance cardiac complications in Ý-thalassemia.  Methods: This study was divided to three groups (group I: 20 healthy subjects, group II:  56 Ý-thalassemic patients, group III: 16 Ý-thalassemic patients with cardiac  complications). The human neopterin and cytokines were determined by ELISA  method, while the measurement of human hs-CRP and Immunoglobulins were  performed using nephelometry. GSTM1 genotype was detected by PCR and cardiac  complications were determined by Echocardiograph. Results: There were statistically  significant increases in Neopterin, IL-6, TNF-Ü, IgA, IgG, IgG subclasses (1, 2, 3 & 4)  and hs-CRP levels, but statistically insignificant difference in IL-4 and IgM in Ý- thalassemic patients compared to normal subjects. Results showed no relation between  GSTM1 null genotype frequency and Ý-thalassemia or cardiac complications  appearance. Also results showed that LV diastolic function was significantly impaired  in cases with GSTM1 null genotype in group III. Conclusion: Neopterin can be used as  a marker for complications of inflammation and infection in Ý-thalassemia. GSTM1  null genotype frequency has no role in beta-thalassemia or cardiac complication  appearance. GSTM1 null genotype has a role in cardiac iron overload and impairing of  left ventricular diastolic function
UR  - http://172.23.153.220/th.pdf
ER  -