A study on the effect of stem cells as compared with dexamethasone on chemotherapy - induced pulmonary fibrosis in rat /
دراسة عن تأثير الخلايا الجذعية فى التليف الرئوى الناجم عن العلاج الكيميائى فى الجرذان و ذلك بالمقارنة بتأثير دواء ديكساميثازون
Ebtehal Mohammad Fikry Elhady ; Supervised Ezz Eldin Aldenshary , Hala M. Fawzy , Marwa M. Safar
- Cairo : Ebtehal Mohammad Fikry Elhady , 2015
- 136 P. : charts , photographs ; 25cm
Thesis (Ph.D.) - Cairo University - Faculty of Pharmacy - Department of Pharmacology and Toxicology
Mesenchymal stem cells (MSCs) have recently emerged as a remedy for various pathological conditions including systemic and pulmonary inflammation. The present study examined the therapeutic effects of bone marrow mesenchymal stem cells (BM - MSCs) and adipose derived mesenchymal stem cells (AD-MSCs) in methotrexate (MTX) - induced pulmonary fibrosis in rats as compared with dexamethasone (Dex.). Methotrexate (14 mg/kg, as a single dose / week for 2 weeks, p.o.) induced lung fibrosis as marked by elevation of relative lung weight, malondialdehyde, nitrite/nitrate, interleukin - 4, transforming growth factor-Ý1, deposited collagen, as well as increased expression of Bax along with the reduction of reduced glutathione content and superoxide dismutase activity. These deleterious effects were antagonize after treatment either with BM - MSCs or AD - MSCs (2106 cells / rat) 2 weeks after MTX to even a better extent than Dex. (0.5 mg / kg / for 7 days, p.o.). In conclusion, BM-MSC and AD-MSCs possessed antioxidant, antiapoptotic, as well as antifibrotic effects which will probably introduce them as remarkable candidates for the treatment of pulmonary fibrosis