Mohamed Abdallah Sayed Ahmed Elweresh <br/><br/>
Biochemical studies on breast cancer /  
 دارسات كيميائية حيوية على سرطان الثدى 
Mohamed Abdallah Sayed Ahmed Elweresh ; Supervised Sanaa Osman Abdallah , Abdelfattah Mohamed Attallah 
 - Cairo :   Mohamed Abdallah Sayed Ahmed Elweresh ,   2015 
 - 176 P. :   charts , facsimiles ;   25cm 
<br/><br/>Thesis (Ph.D.) - Cairo University - Faculty of Science - Department of Biochemistry 
<br/><br/> Breast cancer is considered the leading cause of  cancer death among females in economically developing countries. Tumor  markers are biochemical indicators for the presence of tumor. The epithelial  membrane antigen (EMA) marker could be used to detect small deposits of  malignant cells. This work was concerned with the identification and  quantitative determination of EMA and then estimating its performance as  surrogate marker for identifying breast cancer. Methods: The circulating level  of EMA was determined by Western blot and enzyme linked immunosorbent  assay (ELISA) in sera from 102 women with Breast cancer and 90 women as  controls (40 with benign breast condition and 50 healthy). Results: EMA at  130 kDa was identified. EMA showed area under receiver-operating  characteristic curve (AUC) of 0.946 with 69.6% sensitivity, 94.0% specificity,  96.0% PPV, 60.3% NPV, and 77.6% efficiency. Conclusion: EMA may  improve the identification of breast carcinoma with a high degree of accuracy  in this group of Egyptian women 
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<br/><br/><label>Subjects--Index Terms: </label>Breast cancer Gel electrophoresis Western blotting