Rehab Hamdy Mahmoud Mabrouk  <br/><br/>
Isolation and differentiation of myoblasts from human umbilical cord stem cells and muscles /  
 عزل وتميز الخلايا المخلة للعضلات من الخلايا الجزعية فى الحبل السرى ومن العضلات فى الانسان  
Rehab Hamdy Mahmoud Mabrouk ; Supervised Mahmoud Ahmed Amer , Abeer Ahmed Bahnasy , Mervat Mohamed fouad Eldeftar 
 - Cairo :   Rehab Hamdy Mahmoud Mabrouk  ,   2015 
 - 104 P. :   facsimiles ;   25cm 
<br/><br/>Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Zoology 
<br/><br/> This study was carried out to compare myogenic differentiation of skeletal muscle explants and  cord blood stem cells.  I- In this study, 15 samples of umbilical cord blood were obtained, isolation of mesenchymal  stem cells (MSCs) was done. In vitro expansion of MSCs was performed. MSCs were  propagated in vitro, the myogenic differentiation medium was added. The cells showed a change  in their morphology myotube appeared.  II- Twenty five samples of skeletal muscle (1x1 cm3) were obtained and minced using micro- explant technique .In vitro expansion was performed. Muscle precursor cells reached (75%-80%)  confluence; cells were sub-cultured till myotube formation.  III. RNA extraction was performed; RNA was transformed to (cDNA) and expression of Desmin  and Myo D genes were assessed using RT-PCR technique.    
<br/><br/>
<br/><br/><label>Subjects--Index Terms: </label>Cells and Muscles  Human Umbilical  Myoblasts