Detection and characterization of escherichia coli from different meat sources /
التعرف على ميكروب الايشيريشيا كولاى المعزول من مصادر لحوم مختلفة وتوصيفه
Asmaa Rabea Kamal ; Supervised Kamelia Mahmoud Osman , Mohamed Khaled Elmossalami
- Cairo : Asmaa Rabea Kamal , 2015
- 105 P. : facsimiles ; 25cm
Thesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine Department of Microbiology
Professor of Meat hygiene, Faculty of veterinary medicine, Cairo University In the present study 160 retail meats are examined for isolation of E.coli and the result confirmed biochemically and by using 16srRNA PCR which revealed that there were 55 E.coli isolates recovered from retail meat in percent 34.4% (55\160) with high occurrence in local meat 38.9 %( 44\113) followed by Sudanese meat 29.6% (8\27) and low rate recovered from imported meat 15 %(3\20).Serotyping of recovered isolates revealed that there were high rate for E.coli O121: K64, O86: K61 , in percentage 18 % ( 10\55), 14.5 % (8\55) respectively . and low incidence of O119:K69 in percentage 3.6(2\55).molecular study of antibiotic genes by using PCR with specific primers revealed that there were 74.5 %(41\55) resistant to teta gene (tetracycline) while resistant to erea gene (erythromycin) were 67% (37\55) and the resistant to dhfrV gene (trimethoprime) were 9%(5\55) while resistant to Sul1 gene (sulphatrimethoxaole) were 21.8%(12\51)