Mohamed Mahmoud Mohamed Shaheen <br/><br/>
Cloning of human granulocyte colony stimulating factor in prokaryotic cells /  
 استنساخ معامل حث المستعمرة الحبيبية البشرى داخل خلايا أوليات النواة  
Mohamed Mahamoud Mohamed Shaheen ; Supervised Mohamed Hani Moubasher , Mohamed Ali Saber 
 - Cairo :   Mohmed Mahamoud Mohamed Shaheen ,   2015 
 - 109 P. :   facsimiles ;   25cm 
<br/><br/>Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Botany and Microbiology 
<br/><br/> Human Granulocyte colony-stimulating factor (hG-CSF) is  a growth factor or cytokine produced by a number of different  tissues to stimulate the bone marrow to produce granulocytes  and stem cells. In our study we aimed to optimize production  (expression) of recombinant human G-CSF protein by two  different strategies. The first one is based on the isolation of  natural human G-CSF mRNA from polymorphonuclear cells  (PMNCs). The second one is based on the synthesis of hG-CSF  gene based on the GenBank published sequence. The hG-CSF  open reading frame (ORF) of both strategies were amplified  and cloned into pET-3a expression vector and transformed into  BL21 DE3 E. coli cells for expression of recombinant hG-CSF  protein and was successfully expressed and tested by Western  blot. The results of this work indicate that successful expression  of recombinant hG-CSF protein by two strategies which  confirmed by the molecular weight and the immunogenicity  against the hG-CSF antibodies 
<br/><br/>
<br/><br/><label>Subjects--Index Terms: </label>Granulocyte colony-stimulating factor (G-CSF) Growth  factor,  pET-3a expression system