TY  - BOOK
AU  - Mohamed Mahmoud Mohamed Shaheen
AU  - Mohamed Ali Saber , 
AU  - Mohamed Hani Moubasher , 
TI  - Cloning of human granulocyte colony stimulating factor in prokaryotic cells / 
PY  - 2015///
CY  - Cairo : 
PB  - Mohmed Mahamoud Mohamed Shaheen , 
KW  - Granulocyte colony-stimulating factor (G-CSF)
KW  - Growth  factor,
KW  - pET-3a expression system
N1  - Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Botany and Microbiology; Issued also as CD
N2  - Human Granulocyte colony-stimulating factor (hG-CSF) is  a growth factor or cytokine produced by a number of different  tissues to stimulate the bone marrow to produce granulocytes  and stem cells. In our study we aimed to optimize production  (expression) of recombinant human G-CSF protein by two  different strategies. The first one is based on the isolation of  natural human G-CSF mRNA from polymorphonuclear cells  (PMNCs). The second one is based on the synthesis of hG-CSF  gene based on the GenBank published sequence. The hG-CSF  open reading frame (ORF) of both strategies were amplified  and cloned into pET-3a expression vector and transformed into  BL21 DE3 E. coli cells for expression of recombinant hG-CSF  protein and was successfully expressed and tested by Western  blot. The results of this work indicate that successful expression  of recombinant hG-CSF protein by two strategies which  confirmed by the molecular weight and the immunogenicity  against the hG-CSF antibodies
ER  -