TY  - BOOK
AU  - Mai Moustafa Magdi farrage
AU  - Abeer Ahmed Zayed , 
AU  - Alaa Mohammed Shehab , 
AU  - Hala Saied Zaghloul  , 
TI  - Role of micro RNA as marker of liver injury in  paracetamol acute toxicity / 
PY  - 2015///
CY  - Cairo : 
PB  - Mai Moustafa Magdi farrage , 
KW  - miRNA
KW  - Paracetamol
KW  - RT-PCR
N1  - Thesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Forensic Medicine and Toxicology; Issued also as CD
N2  - Background & objectives: Paracetamol (acetaminophen) overdose is  one of the most common causes of acute liver injury in the world. Early  detection of drug induced liver injury remains a problem as the classic  markers for liver injury lack clinical sensitivity and specificity. MiRNA 122  & miRNA 192 are promising biomarkers with high sensitivity and  specificity for detection of drug induced liver injury. Aim of this study: The  aim of the current study is to investigate the potential utility of miRNAs 122  & 192 as markers of human paracetamol-induced liver injury and to  compare miRNA with existing biomarkers of liver injury in humans. Results: The age of all participants ranges between 11-57 years with  mean age in group (1) 24.83, in group (2) was 23.2 and in group (3) it was  31.07. We found that 100% of cases were suicidal. Males were 21.4% and  females were 78.6% of all studied cases. The mean and standard deviation of  paracetamol level (mg/l) in group (1) was 287.34±79.74 and in group (2) it  was 31.71±12.67. The mean and standard deviation of liver enzymes (AST  & ALT) (IU/L) in the study group (1) was in normal range (31.31±2.71 &  20.57±1.96 respectively). The mean and standard deviation of liver enzymes  (AST & ALT) in the study group (2) was in range higher than normal limits  (91.80±26.93 & 39.29±13.77respectively). Results of paracetamol level,  miRNA 122 levels and miRNA 192 levels showed statistical significance  between the study group G3 and both groups of cases (groups G1 & G2).  Conclusion: Both types of miRNAs included in the study (122 & 192),  are highly sensitive markers of paracetamol induced liver injury in cases  both with early and late presentation.  Keywords: paracetamol, miRNA, RT-PCR, Drug induced liver injury
ER  -