Effect of genetic polymorphisms of CYP P450 and multidrug resistance 1 on cyclosporine after renal transplantation in Egyptian patients /
د ا ا وم ٤٥٠ و اواء ١ رر ا ا ت زرع ا
Yasser Ramadan Abdalwanees Hashad ; Supervised Ahmad M. Farag , Amr Saad Mohamed , Enas A. Ellateef
- Cairo : Yasser Ramadan Abdalwanees Hashad , 2015
- 229 P. : facsimiles ; 25cm
Thesis (Ph.D.) - Cairo University - Faculty of Science - Department of Biochemistry
This study was conducted to to evaluate retrospectively the effects of genetic polymorphisms of CYP3A4, CYP3A5 and MDR1 on cyclosporine trough and 2 hour s blood concentration during the early period after renal transplantation in Egyptian patients. Forty two renal transplant recipients and forty eight healthy controls Egyptian subjects were genotyped by polymerase chain reactionrestriction fragment length polymorphism for CYP3A4*18A, CYP3A5*3 and MDR1 C3435T. Cyclosporine C₀ and C₂ whole blood levels were measured. The frequency of the CYP3A4*18A, CYP3A5*3 and MDR1 C3435T variant alleles in all subjects were 0.006, 0.865 and 0.344 , respectively,comparison of the CsA levels between patients with CYP3A5 3*/3* genotype and those with 1*/1* genotype, showed that all levels of C₀ and C₂ are higher in 3*/3* genotype than that of 1*/1* genotype but without significant differences between the two groups at day-7 C₀ (P = 0.25), day-30 C₀ (p = 0.28), day-90 C₀ (p = 0.3) and at day-30 C₂ (P = 0.64)