TY  - BOOK
AU  - Rabab fouad Mohamed
AU  - Bothaina Madkour , 
AU  - Hala Farawela , 
AU  - Nariman Mohamed Zahran , 
TI  - Fibronectin gene polymorphisms in HCV related type II mixed cryoglobulinemia: risk of development of B-cell lymphoma / 
PY  - 2016///
CY  - Cairo : 
PB  - Rabab fouad Mohamed , 
KW  - FN gene polymorphisms
KW  - HCV
KW  - NHL
N1  - Thesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Clinical and Chemical Pathology; Issued also as CD
N2  - NHLs are clonal malignancies with diverse clinical presentations, pathogenesis  and biologic behavior. It results from accumulation of genetic insults that lead to  unregulated lymphocyte proliferation. Malignant lymphocytes usually accumulate  in lymph nodes and cause the characteristic clinical feature of lymphadenopathy.  HCV is a major cause of liver-related morbidity and is increasingly recognized as  an instigator of B-cell lymphoproliferative disorders such as mixed  cryoglobulinemia and non-Hodgkin lymphoma. Fibronectin is a multifunctional  glycoprotein of 250 kDa which is encoded by a gene of over 75 kb in length,  located on chromosome 2q34{u2013}36 and composed of 50 exons. The purpose of the  current study was to investigate the possible relationship between two FN  polymorphisms (called MspI and HaeIIIb) and the development of lymphoma in  HCV positive patients with mixed cryoglobulinemic syndrome compared to  healthy age and sex matched individuals. The present study included 75 patients;  25 HCV positive patients with MCns and 50 NHL patients, in addition to 25  healthy controls. To achieve this aim, genotyping for FN gene was done by PCR- RFLP technique and screening for HCV infection was performed by ELISA and  confirmed by RT-PCR, cryoglobulins were detected by cold precipitation (4{u00B0}C for  72{u2013} 96 h) and plasma FN levels were assessed by ELISA. The study revealed that  HaeIII-AB genotype can be considered as a risk factor for NHL development and  high HaeIII-A allele frequency seems to be linked to NHL development. It was  also found that plasma FN level did not show any significant difference between  NHL patients and each of HCV positive patients with MCns and controls
UR  - http://172.23.153.220/th.pdf
ER  -