TY  - BOOK
AU  - Alzahraa Muhammad Abdelrasoul Abdelatty
AU  - Fathy Farouk Mohamed  , 
AU  - Mohamed Ahmed Tony , 
AU  - Richard Arthur Erdman , 
TI  - Short term dietary modifications alter mammary lipogenic gene expression in mid lactating dairy cow using novel non-invasive  rna extraction technique / 
PY  - 2016///
CY  - Cairo : 
PB  - Alzahraa Muhammad Abdelrasoul Abdelatty , 
KW  - CLA
KW  - Cytosolic crescent
KW  - Dairy cow
N1  - Thesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Nutrition; Issued also as CD
N2  - Milk fat is the most variable component of milk, it is easily  changed by nutritional and environmental factors rendering it a central  point of interest for the daily market and scientists. Dietary  manipulation can have a profound influence on milk fat synthesis and  its fatty acid profile. However, our basic understanding of how dietary  manipulations regulates mammary lipogenesis is limited by the use of  mammary biopsy to obtain representative tissue sample for gene  expression analysis, as repeated biopsies over short time interval from  the same animal is not possible. Therefore, the general objective of this dissertation was to  measure the sequential changes in milk fat concentration, milk fatty  acid profile and mammary lipogenic gene expression that occur during  the transition from normal to dietary induced either elevated or  depressed milk fat concentration, using new non-invasive alternative  technique by extracting RNA from cytosolic crest trapped in large fat  globules. Two experiments were conducted: 1) feed restriction that  induce negative energy balance, body fat mobilization, and elevated  milk fat concentration, 2) dietary induced milk fat depression by the  use of dietary CLA that resulted in depression in milk fat  concentration.
ER  -