TY  - BOOK
AU  - Marwa Mohammed Abdelgwad
AU  - Dina Sabry Abdelfattah , 
AU  - Samar Ali Marzouk , 
AU  - Tamer Mahmoud Elbaz , 
TI  - Biomarkers assessment for detection of hepatocellular carcinoma / 
PY  - 2016///
CY  - Cairo : 
PB  - Marwa Mohammed Abdelgwad , 
KW  - Alpha fetoprotein -L3
KW  - Golgi protein 73
KW  - HCC
N1  - Thesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Medical Biochemistry; Issued also as CD
N2  - We prospectively  collected 50 control subjects (group I), 70 HCV patients (group II) and  100 HCC patients (group III) from HCC multidisciplinary clinic (Kasr El- Eini Hospital, Cairo University, Egypt). AFP was measures as proteinby  ELISA. AFP and GP73 gene expression were assessed by quantitative  reverse transcriptase-polymerase chain reaction (qRT-PCR).Also, AFP- L3 and GP73 serum levels were measured by Western blot technique.  Patients' life survivals were calculated. Results: mRNA expression of  AFPand GP73 genes in HCC patients [(2.01+0.87) and (4.32+1.56)] were  significantly higher than control group [(0.15+0.02) and (0.25+0.03)]&  HCV group [(0.86+0.47) and (1.50+0.73)].Protein levels of AFP-L3 and  GP73in HCC patients[(11.56+3.85) and (21.75+7.12)], were significantly  higher than control group [(0.80+0.36) and (1.40+0.59)] and HCV group  [(2.62+1.33) and (7.60+2.90)]. Univariate analysis revealed that up- regulated AFP and GP73 gene expression and AFP-L3 and GP73 protein  levels were significant prognostic factors for HCC (p < 0.001). Besides,  number of focal lesion and focal lesion size were significant prognostic  factors for HCC. Concerning multivariate analysis, up-regulated AFP  gene expression and AFP-L3 and GP73 protein levels were significant  prognostic factors for HCC (p=0.039,p<0.001, and p=0.039     XI    respectively).In addition, focal lesion size (p<0.001) was significantly  prognostic factor for HCC. ROC curve comparing AFP, AFP-L3  andGP73 protein levels, showed that GP73 had the highest sensitivity  (100%),and followed by AFP-L3 (94.7) and finally AFP with the lowest  sensitivity (71.1%).  Conclusions: GP73 and AFP-L3 are more sensitive markers than AFP  for early detection of HCC patients. Also, both proved to be essential  prognostic factors in univariate and multivariate analyses.  Key words: Alpha fetoprotein -L3, Golgi protein 73, HCC, HCV
UR  - http://172.23.153.220/th.pdf
ER  -