TY  - BOOK
AU  - Dina Mahmoud Abbas Elkersh 
AU  - Fatma Salem Elsakhawy , 
AU  - Mohamed  Mohey Eldin Elmazar , 
AU  - Riham Salah Eldine Elsayed , 
TI  - A Phytochemical and biological study of markhamia zanzibarica (bojer ex DC.) K. schum. cultivated in Egypt / 
PY  - 2016///
CY  - Cairo : 
PB  - Dina Mahmoud Abbas Elkersh , 
KW  - DNA fingerprint
KW  - GC/MS
KW  - Markhamia zanzibarica
N1  - Thesis (Ph.D.) - Cairo University - Faculty of Pharmacy - Department of Pharmacognosy; Issued also as CD
N2  - Abstract Authentication and characterization of Markhamia zanzibarica (Bojer ex DC.) K. Schum through DNA fingerprint. The study of lipoidal matter of n-hexane extract of the  unsaponifiable matter and fatty acid methyl ester of M. zanzibarica were analyzed using  GC/MS. Isolation of oleanolic acid, ursolic acid, quadrangularic acid L and quadrangularic acid  K from the chloroform fraction and isolation of 5,7,3`,5` tetrahydroxy flavanone, luteolin, Nigaichigoside F , luteolin-7-O-{uF062}-D-glucoside and apigenin-7-O-rutinoside from the ethyl  2 acetate fraction. Total steroidal content, total phenolic content and total flavonoid content were carried out on the total alcoholic extract and antioxidant assay using DPPH assay which was carried  out on different extracts and fractions of aerial parts of Markhamia zanzibarica. The in vitro cytotoxic activity revealed that n- hexane extract was the most potent cytotoxic one on the HeLa cancer cell line while the Chloroform fraction was the most potent cytotoxic one on the MCF-7 cancer cell line. The in vitro estrogenic and anti-estrogenic activities revealed that the defatted alcoholic extract and the ethyl acetate fraction possessed estrogenic activity whereas the n-hexane extract and n-butanol fraction possessed anti-estrogenic activity. The study of lipoidal matter revealed presence of sterols in the n-hexane extract while the study of LC-MS/MS revealed presence of verbascoside compounds in the n-butanol fraction
UR  - http://172.23.153.220/th.pdf
ER  -