TY  - BOOK
AU  - Mohamed Ramadan Sayed Nasr Elshaer 
AU  - Hanan Mohamed Shafik , 
AU  - Mervat Sayed Mohamed , 
TI  - Development of radioimmunoassay system for measurement of testosterone in human serum / 
PY  - 2016///
CY  - Cairo : 
PB  - Mohamed Ramadan Sayed Nasr Elshaer  , 
KW  - Antibody
KW  - Radioimmnuoassay
KW  - Testosterone
N1  - Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Biochemistry; Issued also as CD
N2  - In the present study, development of a testosterone radioimmunoassay (RIA) technique using liquid phase double antibody is the main objective. The production of polyclonal testosterone antibody was undertaken by immunizing  seven females white New-Zealand rabbits. The preparation of radio-iodinated testosterone tracer was carried out. A set of testosterone standards was prepared  using highly purified testosterone antigen. Formulation, optimization and validation of the local testosterone double antibody liquid phase RIA system were carried out.The result obtained show that the RIA system of testosterone is  sensitive, specific and accurate and can be used in diagnosis of variety of diseases especially hypogonadism and possible male infertility
ER  -