TY  - BOOK
AU  - Amr Yusuf Abdellatif 
AU  - Heba Nabil Baz , 
AU  - Mohamed Shehata Mohamed Abdallah , 
AU  - Wael Faek Gamal Eldeen , 
TI  - Association between single nucleotide polymorphism (C677T) in MTHFR gene and insulin resistance in polycystic ovary syndrome / 
PY  - 2016///
CY  - Cairo : 
PB  - Amr Yusuf Abdellatif , 
KW  - C677T -  -
KW  - MTHFR gene
KW  - Polycystic ovary syndrome
N1  - Thesis (M.Sc.) - Cairo University - Faculty of Medicine - Department of Clinical and Chemical Pathology; Issued also as CD
N2  - Background: Polycystic ovary syndrome is the most common endocrinopathy affecting females of reproductive age, Features include  hyperandrogenism, chronic anovulation and polycystic ovaries on  ultrasound. insulin resistance is one of the major consequences affecting women with pcos.  Aim of the study: The aim of the present work is to study  Association between single nucleotide polymorphism (C677T) in MTHFR gene and Polycystic ovary syndrome in patients with or without  insulin resistance in comparison to normal healthy controls. Subjects and methods: the study was conducted on eighty patients  recruited from outpatient clinic of obstetrics and gynecology department  in Kasr Alainy hospital. In addition to 50 sex and age comparable  subjects who were taken as a control group.clinical examination,  ultrasound and laboratory analysis including total testosterone,fasting and  post prandial glucose and insulin and C677T polymorphism in MTHFR  gene was detected using polymerase chain reaction restriction fragment  length polymorphism PCR-RFLP. Results: Regarding the prevalence of MTHFR C677T genotype  among our cases and the corresponding controls, the frequency of the CC,  CT and TT was 57.5%, 41.2% and 1.2% respectively in cases, and 70%,  30.0% in the controls respectively.  There is no association between single nucleotide polymorphism (C677T) in MTHFR gene and Polycystic ovary syndrome. Regarding the association between C677T and insulin resistance  there is significant difference between mutant variant of C677T of  MTHFR gene and other alleles in insulin resistant subjects regardless of  PCOS patients
ER  -