Noura Hassan Mohamed  <br/><br/>
Purification, antiserum production, serological and molecular detection of Cucurbit Yellow Stunting Disorder Virus (CYSDV) in Egypt /  
 تنقيــة وإنتـاج المصـل واختبـارات سيـرولوجيـة وجـزيئـيـة  لفيروس إصفرار وتقزم القرعيات في مصر                       
Noura Hassan Mohamed ; Supervised Gamal Amin Mohamed Ghanem , Ahmed Ismail Abdelalim  
 - Cairo :   Noura Hassan Mohamed ,   2016 
 - 120 P. :   facsimiles ;   25cm 
<br/><br/>Thesis (Ph.D.) - Cairo University - Faculty of Agriculture - Department of Plant Pathology and physiology 
<br/><br/> Cucurbit yellow stunting disorder crinivirus (CYSDV) causes significant yield losses in field- and in greenhouse grown cucurbits in Egypt. Evalution of cucumber varietal reaction against CYSDV-infection using the induced antibody (CYSDV-Pab) in DAS-ELISA. The induced polyclonal antibody (CYSDV-Pab) was also evaluated in detecting CYSDV antigen in the infected plants through serological tests. Reverse transcription-polymerase chain reaction (RT-PCR) techniques was developed to detect CYSDV that target either the coat protein gene or p22. RT-LAMP was reliable for diagnosis of CYSDV-infected leaf samples and insect vectors from the field in 60 min 
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<br/><br/><label>Subjects--Index Terms: </label>Antiserum production  CYSDV DBIA