TY - BOOK AU - Noura Hassan Mohamed AU - Ahmed Ismail Abdelalim, AU - Gamal Amin Mohamed Ghanem , TI - Purification, antiserum production, serological and molecular detection of Cucurbit Yellow Stunting Disorder Virus (CYSDV) in Egypt / PY - 2016/// CY - Cairo : PB - Noura Hassan Mohamed , KW - Antiserum production KW - CYSDV KW - DBIA N1 - Thesis (Ph.D.) - Cairo University - Faculty of Agriculture - Department of Plant Pathology and physiology; Issued also as CD N2 - Cucurbit yellow stunting disorder crinivirus (CYSDV) causes significant yield losses in field- and in greenhouse grown cucurbits in Egypt. Evalution of cucumber varietal reaction against CYSDV-infection using the induced antibody (CYSDV-Pab) in DAS-ELISA. The induced polyclonal antibody (CYSDV-Pab) was also evaluated in detecting CYSDV antigen in the infected plants through serological tests. Reverse transcription-polymerase chain reaction (RT-PCR) techniques was developed to detect CYSDV that target either the coat protein gene or p22. RT-LAMP was reliable for diagnosis of CYSDV-infected leaf samples and insect vectors from the field in 60 min UR - http://172.23.153.220/th.pdf ER -