TY  - BOOK
AU  - Doaa Mahdy Mohamed Mahmoud Elwakel 
AU  - Eman Ahmed Elseidi , 
AU  - Hanaa Ibrahim Rady , 
AU  - Reham Ali Dwedar , 
TI  - Human metapneumovirus pediatric respiratory infections : : Comparing direct immunofluorescence versus polymerase chain reaction / 
PY  - 2016///
CY  - Cairo : 
PB  - Doaa Mahdy Mohamed Mahmoud Elwakel , 
KW  - Direct immunofluorescence
KW  - Human metapneumovirus
KW  - Polymerase  chain reaction
N1  - Thesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Microbiology and Immunology; Issued also as CD
N2  - Human metapneumovirus has been recognized as a common cause of respiratory  infections particularly in infants and young children. The aim of the study is to  determine the frequency of hMPV infections among children less than 2 years of age and  to define its clinical features and seasonal pattern over a single respiratory season. Direct  fluorescent antibody (DFA) assay was compared to RT-PCR for the detection of hMPV  in respiratory samples. Fifty pediatric patients presenting with acute respiratory tract  infection were enrolled in the study. Nasopharyngeal aspirate specimens were subjected  to DFA assay and real-time PCR for the presence of hMPV. It was found that hMPV  accounted for 4 cases (8%) of the studied patients and the infection rate was higher  (20%) among the 12{u2013}24 months age group. Moreover, hMPV-positive cases were  mostly detected in winter months. Their main clinical features were respiratory distress  (100%) and cough (100%), with pneumonia (75%) and bronchiolitis (25%) being the  main clinical diagnoses among the studied patients. By considering the real-time PCR  assay as the gold standard test for diagnosing hMPV, the sensitivity, specificity, PPV,  NPV and test efficiency of DFA for the detection of hMPV were 50%, 100%, 100%,  95.8% and 96%, respectively. Direct fluorescent antibody assay for hMPV was a highly  specific and simple test but of low sensitivity
UR  - http://172.23.153.220/th.pdf
ER  -