TY  - BOOK
AU  - Hadeel Mosfer Dakhil Aljaeed
AU  - Amira Abutaleb , 
AU  - Mohamed Hani Moubasher , 
TI  - Molecular characterization between aflatoxinogenic and non- aflatoxinogenic strains of aspergillus flavus and aspergillus parasiticus  / 
PY  - 2016///
CY  - Cairo : 
PB  - Hadeel Mosfer Dakhil Aljaeed , 
KW  - Aflatoxinogenic and non- aflatoxinogenic strains
KW  - Aspergillus flavus and aspergillus parasiticus
KW  - Molecular characterization
N1  - Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Botany and Microbiology; Issued also as CD
N2  - Not all A. flavus and A. parasiticus are aflatoxigenic. - Eight strains were aflatoxigenic and three were non aflatoxigenic out of eleven strains. Five strains of aflatoxinogenic fungi were A.  flavus var. columnaris, two strains were A. parasiticus, and one strain was A. flavus. Three isolates were n o n-aflatoxinogenic. Two of which were A. flavus var columnaris and one A. flavus. - All aflatoxin-producing strains could release both aflatoxin B and G groups - Aflatoxin production was affected by broth type as shown in A. flavus  var. columnaris strains 9022, 9024, 9025, 9030, 9031 A. flavus strain  9027 and A. parasiticus strains 9029, 9032. - In spite of, TLC distinguished between aflatoxin-producing and  non-producing strains, it failed in some cases to detect aflatoxin- producing strains because instability of aflatoxin production may occur in certain toxinogenic strains.  - Multiplex RT-PCR for the selected genes also showed a good  correlation between aflatoxin gene expression and aflatoxin production. - Expression of nor-1 (Afl-D), omt- B (Afl-O), omt-A ((Afl-P)) and ord-A  (Afl-Q) genes provided useful differentiation of aflatoxigenic and non- aflatoxigenic strains of A. flavus and A. parasiticus. - The expression of the selected genes varied with regard to the aflatoxin- producing ability and types of media
UR  - http://172.23.153.220/th.pdf
ER  -