TY  - BOOK
AU  - Marian Nagui Nazeer Guirguis 
AU  - Ahmed Alsayed Altaweel , 
AU  - Walaa Ahmed Rabie , 
AU  - Walaa Alsharaany , 
TI  - Genetic impact of erythrocyte complement receptor 1(ECR1) A3650g polymorphism on neonatal  respiratory distress syndrome in egyptian neonates / 
PY  - 2016///
CY  - Cairo : 
PB  - Marian Nagui Nazeer Guirguis , 
KW  - CR1
KW  - Gene polymorphism
KW  - RDS
N1  - Thesis (M.Sc.) - Cairo University - Faculty of Medicine - Department of Clinical and Chemical Pathology; Issued also as CD
N2  - Background: Complement receptor 1is a membrane-bound single-chain glycoprotein. Its expression on erythrocytes contributes to immune complex clearance and transfer of C3b- opsonized antigens to splenic and hepatic macrophages. Respiratory distress syndrome (RDS) is a relatively common syndrome characterized by shortness of breath and respiratory failure. In premature infants RDS develop because of impaired surfactant synthesis and secretion  with resultant hypoxemia and hypercarbia. Aim: was to assess the association between genetic polymorphisms of Eryrthocyte Complement receptor1 gene and the susceptibility to Neonatal respiratory distress syndrome (NRDS) in Egypt. Methods: This study was carried out on 50 neonates <37 weeks gestational age presenting  with RDS and 50 cross matched neonates without RDS as control group. Two gene  polymorphisms were studied: Single nucleotide polymorphism (SNP) was analyzed by real- time PCR (RT-PCR) allelic discrimination analysis of A3650G SNP (rs2274567) of  ECR1gene using TaqMan SNP genotyping , while genotyping of the other SNP ( HindIII  digested ECR1) alleles was done using restriction fragment length polymorphism( PCR- RFLP). Results: The results of this study revealed that birth weight, gestational age and Apgar score at 5 min (p-value<0.001) are statistically significant factors in development of RDS. However, maternal risk factors, mode of delivery, Alleles A,G,H were found to be non- statistically significant factors. However, Alelle L was found to be significant statistically in  severity of RDS grade (p-value=0.004)
ER  -