TY - GEN AU - Ahmed Adel Attia Seada AU - Nashwa Abdelsalam Ezz_Eldeen , AU - Rafik soliman , TI - Preparation of monoclonal antibodies against tetanus toxin and evaluation of its neutrelaizing potential / PY - 2006/// CY - Cairo : PB - Ahmed adel attia seada , KW - antibodies KW - Monoclonal KW - Tetanus toxin N1 - Thesis (M.Sc.) - Cairo University - Faculty Of Veterinary Medicine - Department Of Microbiology , Bacteriology , Mycology and Immunology; Issued also as CD N2 - Preparation of monoclonal antibodies against tetanus toxin and evaluation of its efficacy obtained by using spleen cells (B - Cells) which harvested from well immunized BALB / c mice were used , also myeloma cell lines P3NS / 1 were usedFusion between spleen cells and myeloma cell lines occurred through fusogenic material called PEG 1500 , 40in hundred concentration to produce hybridomas which had the characteristic of both parents the ability to grow indefinitely from myeloma and the ability to produce of specific antibody from the B - cellsIn this study we succeed to produce five hybridomas producing monoclonal antibodies against tetanus toxin and also succeed to be cloned into 5 stable clones producing monoclonal antibodies against tetanus toxin UR - http://172.23.153.220/th.pdf ER -