TY  - BOOK
AU  - Amal Mohamed Ibrahim Osman
AU  - Emtithal Mohammed Abdesamiea , 
AU  - Hamdy Taher Mohamed Abouelenain , 
AU  - Mona Abdelaziz Mohamed , 
TI  - Diagnosis of honeybee diseases using PCR and laser spectroscopic techniques / 
PY  - 2015///
CY  - Cairo : 
PB  - Amal Mohamed Ibrahim Osman , 
KW  - Carniolan and Italian honeybee races
KW  - P. l. larvae
KW  - PCR
N1  - Thesis (M.Sc.) - Cairo University - National Institute of Laser Enhanced Science - Department of Laser Application in Environmental Metrology Photochemistry and Agriculture; Issued also as CD
N2  - Paenibacillus larvae subsp. larvae, the causative pathogenic  bacterium of virulent brood disease (American foulbrood, AFB).  DNA extracted from P. l. larvae strain was subjected to PCR using  specific primers of a partial sequence of 16S rRNA gene that closely  related to the bacterium. Assayed bacterial strain produced a defined  band of 700 bp in size. This test was done based on DNA {u2013} extraction  of bacterial colonies and different larval stages(1-5 days, of age) from  Carniolan (Apis mellifera carnica) honeybee and Italian (Apis  mellifera ligustica), The results of PCR analysis showed that the  (day1) of all honeybee races didn't amplify the pathogen fragment  (700bp) and all larval stages from (day2- day5) generated PCR  fragment of 700bp. Also, this defined fragment was detected in  larvae, pupae, adult worker and virgin queen resulted from the  grafting Italian honeybee. On the other hand, the physicochemical  structure of genomic DNA of pathogenic bacterium P. l. larvae and  the two honeybee races before (-ve control) &after infection with the  pathogen were identified and characterized qualitatively and  quantitatively using molecular laser Raman (MLRS) and infra-red  (FTIR) spectroscopies for the first time. The results of spectroscopic  analysis clearly revealed that the pathogen was more abundant in  Italian race from the early infection stage (day1) to (day5) of age.  than Carniolan which showed more resistant to pathogen infection of  the (day2)
UR  - http://172.23.153.220/th.pdf
ER  -