A Phytochemical Study of Certain Egyptian Plants with Antioxidant Activity /
دراسة فيتوكيميائية لبعض النباتات المصرية التى لها تأثير مضاد للأكسدة
Dalia Mohamed Rasheed ; Supervised Kamilia Foli Taha , Nebal Darwish El Tanbouly , Hala El Hefnawy
- Cairo : Dalia Mohamed Rasheed , 2006
- 136P : ill ; 30cm
Thesis (M.Sc.) - Cairo University - Faculty Of Pharmacy - Department Of Pharmacognosy
These extracts were tested in vivo on lipid peroxidation in ethanol - CCl4 induced liver toxicity model EtOAc fraction of these two extracts was proved to be responsible for the antioxidant activity by DPPH assay , and it was further purified via polyamide , cellulose and sephadex LH - 20 column chromatography Antioxidant compounds were isolated and identified through spectroscopic methods as (quercitrin) and (isoquercitrin) in Pgraveolens extract , (reynoutrin) , (rutin) and gallic acid in C siliqua extract Furthermore , standardization of both plant extracts was carried out by determination of the total polyphenol content using Folin - Ciocalteu colorimetric method Total flavonoid content was determined by spectrophotometric and HPLC methods