TY  - BOOK
AU  - Ghada Hamdi Elsayed Mohamed 
AU  - Gamal Abdelmegeed Abdelghany , 
AU  - Mervat Sayed Mohamed , 
AU  - Rafat Milad Mohareb , 
TI  - Effect of some newly synthesized steroid  derivatives on apoptosis of breast cancer cells / 
PY  - 2017///
CY  - Cairo : 
PB  - Ghada Hamdi Elsayed Mohamed , 
KW  - Breast cancer
KW  - Heterocycles
KW  - Steroids
N1  - Thesis (Ph.D.) - Cairo University - Faculty of Science - Department of Biochemistry; Issued also as CD
N2  - Anticancer agents consisting of hybrid molecules are used to improve effectiveness and diminish drug resistance. The current study aimed to introduce newly synthesized hetero-steroids of promising anticancer effects. Besides, the pro-apoptotic effects of new compounds were investigated extensively. Several pyrimidino-, triazolopyrimidino-, pyridazino-, and curcumin- steroid derivatives were synthesized, elucidated and confirmed using the spectral and analytical data. The synthesized hetero-steroids, compounds 9, 10, 11, 12, 13, 14, 15, 18, 20, 21, 22 and 24, were tested for their cytotoxic effects versus human breast cancer cells (MCF-7) using neutral red supravital dye uptake assay. Compound 24 (IC50 = 18 æM) showed more inhibitory influence on MCF-7 growth. Using QRT-PCR (Quantitative real time-polymerase chain reaction), CCND1, Survivin, BCL-2, CDC2, P21 and P53, genes expression levels were investigated. The study results disclose that compounds 4, 7, 18, 24 knocked down the expression levels of CCND1, Survivin, BCL-2 and CDC2. However, P21 and P53 were up- regulated by compounds 21, 22. This study introduced promising pro-apoptotic anticancer agents acting through the modulation of key regulators of apoptosis and cell cycle genes. The current study revealed the most promising compounds 7, 18, 21 down-regulated miR-34a, miR-98 and miR-214 expression levels and decreased upon drug resistance response.
UR  - http://172.23.153.220/th.pdf
ER  -