Assessment of the role of PPAR-Þ2 Pro12Ala polymorphism and D2 Thr92Ala polymorphism in type 2 diabetic patients /
فى مرضى النوع الثانى من داء السكرى D2 Thr92Ala و تعدد الأشكال الجينية ل PPAR-Þ2 Pro12Ala تقييم دور تعدد الأشكال الجينية ل
Ghada Mohamed Ahmed Abdelwahab ; Supervised Mohammed Shehata , Mona Fathy
- Cairo : Ghada Mohamed Ahmed Abdelwahab , 2017
- 111 P. : charta ; 25cm
Thesis (M.Sc.) - Cairo University - Faculty of Medicine - Department of Clinical and Chemical Pathology
Background: Peroxisome proliferator-activated receptor gamma 2 (PPAR-Þ2) is a transcription factor with a key role in adipocyte differentiation, lipid storage and glucose homeostasis. The Ala allele of the common Pro12Ala polymorphism in the isoform PPAR-Þ2 is at the center of many controversies because in some populations, it has been observed to be associated with T2DM or obesity but, not in others. Type 2 deiodinase (D2) is an enzyme responsible for the conversion of T4 to T3. The Thr92Ala polymorphism has been shown related to an increased risk for developing type 2 diabetes mellitus (T2DM). The aim of this study was to investigate the association of Pro12Ala polymorphism in the PPAR-Þ2 gene and Thr92Ala polymorphism in the D2 gene and type 2 diabetes mellitus. Subject and methods: This case-control study included 80 T2DM patients and 82 controls all unrelated. Genotyping of PPAR-Þ2 (Pro12Ala) and genotyping of D2 (Thr92Ala) polymorphisms were examined by using the restriction fragment length polymorphism -polymerase chain reaction (PCR-RFLP). Results: The frequency of the mutant homozygous of PPARG gene was 7.5% among T2DM and 6.1% among controls, while the frequency of the mutant homozygous of D2 gene was 15% among T2DM and 9.8% among controls. The genotype analysis of PPARG gene polymorphism, and D2 gene polymorphism revealed no statistically significant difference between the two studied groups, for PPARG (P=0.937), and for D2 (P=0.572). Conclusion: PPARG Pro12Ala polymorphism, D2 Thr92Ala polymorphism may not be associated with T2DM