TY  - BOOK
AU  - Sara Mohamed Abdelkhalek Abdelrazek
AU  - Abeer Ahmed Bahnassy , 
AU  - Amira Mohamed Raafat , 
AU  - Mahmoud Ahmed Amer , 
TI  - Assessment of the trans-differentiation potential of bone marrow and umbilical cord blood stem cells in generating insulin producing cells / 
PY  - 2016///
CY  - Cairo : 
PB  - Sara Mohamed Abdelkhalek Abdelrazek , 
KW  - Bone marrow
KW  - Insulin producing cells
KW  - Umbilical cord blood stem cells
N1  - Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Zoology; Issued also as CD
N2  - This study was carried out to assess the feasibility of generating insulin-producing cells from progenitor cells obtained from the bone marrow (BM) and cord blood (CB) and compare the trans-differentiation potential of the two studied sources (BM versus CB cells). I- In this study, 20 samples of umbilical cord blood were obtained, isolation of mesenchymal stem cells (MSCs) was done. In vitro expansion of MSCs was performed. MSCs were propagated in-vitro, the insulin-producing cells differentiation medium was added. The cells showed a change in their morphology insulin-producing cells appeared. II- Twenty samples of rat bone marrow were obtained; isolation of mesenchymal stem cells (MSCs) was done. In vitro expansion of MSCs was performed. MSCs were propagated in vitro, the insulin-producing cells differentiation medium was added. The cells showed a change in their morphology, and became insulin-producing cells appeared. III. RNA extraction was performed; RNA was transformed to (cDNA) and expression of GAPDH, Insulin genes were assessed using RT-PCR technique
UR  - http://172.23.153.220/th.pdf
ER  -