TY  - BOOK
AU  - Reem Mohamed Ahmed Embaby
AU  - Hanan Ramadan Nassar , 
AU  - Laila Abdelrahman Hegazy , 
AU  - Nohair Soliman , 
TI  - Clinical role of micro-rnas expression in acute myeloid leukemia : : miR-155, miR-424 deregulation, correlation With NPM1 / 
PY  - 2017///
CY  - Cairo : 
PB  - Reem Mohamed Ahmed Embaby , 
KW  - Acute Myeloid Leukemia
KW  - miR-155
KW  - miR-424
N1  - Thesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Clinical and Chemical Pathology; Issued also as CD
N2  - AML is a highly heterogeneous disease characterized by failure of myeloid precursor cells to undergo terminal differentiation from proliferating precursor cells into mature blood cells. MicroRNAs (miRNAs) are small, non-coding RNAs control the expression of a number of genes involved in commitment and differentiation of hematopoietic stem cells and tumorigenesis. The study aimed to clarify role of miR-155,mir-424,in pathogenesis ,prognosis of AML and correlation of both miRNAs with NPM1 gene .The present study done on 40 adult newly diagnosed AML cases that were taken from National Cancer Inistitute & medical oncology unit Kasr Aini School of Medicine, Cairo University and 40 sex & age matched controls. A quantitative reverse-transcription PCR (qRT-PCR) assay was done to relatively quantify miR-424,miR-155. (RT-PCR) assay was done to assess NPM1 mutation and correlate it with both micro-RNAs.There was highly statistically significant difference in miR-424 between both patients and controls (p=0.0001),miR 155 was a bad predictor for AML cases. No correlation was found between miR -424, miR-155 and NPM1 .So that deregulated expression of both miRNAs does not depend on NPM1 to exhibit its role in leukemogenesis
UR  - http://172.23.153.220/th.pdf
ER  -