TY  - BOOK
AU  - Sara Elhossain Aly Reda Mohamed
AU  - Abdelmeguid Kassem , 
AU  - Lamiaa Abdelfattah Ahmed Madkour , 
AU  - Mona Abdelwahab Abdemessih , 
TI  - Comparative study between different laboratory methods in the diagnosis of human brucellosis / 
PY  - 2017///
CY  - Cairo : 
PB  - Sara Elhossain Aly Reda Mohamed , 
KW  - Brucellosis
KW  - ELISA
KW  - qPCR
N1  - Thesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Microbiology and Immunology; Issued also as CD
N2  - The aim of the study was to compare the performance of different serological tests in the diagnosis of brucellosis, considering quantitative real time PCR (qPCR) as the reference test. Quantitative real time PCR was positive for Brucella DNA in 65% out of 100 serum samples. Both the standard tube agglutination test (STAT) and rose bengal (RB) test revealed high sensitivity in the detection of brucellosis (95% and 89% respectively). The positive predictive value (PPV) of STAT was 65%, while RB and ELISA had the same PPV (64%). On the other hand, the negative predictive values were 40%, 22% and 33% for STAT, RB and ELISA respectively. However, the specificity of both STAT and RB was surprisingly low (6%). On the other hand, ELISA had a sensitivity of 75% and a specificity of 23%. These results highlight the value of ELISA as a rapid and reliable tool for the diagnosis of brucellosis particularly in endemic areas
UR  - http://172.23.153.220/th.pdf
ER  -