Asmaa Sami Mohammed Mansour <br/><br/>
Unmodified gold nanoparticles for recognition of enterotoxin genes and other genes of Staphylococcus aureus isolated from bovine milk /  
 جزيئات النانو للذهب الغير معدلة للتعرف على جينات السموم المعوية و جينات أخرى للميكروب العنقودى الذهبى المعزول من ألبان الأبقار 
Asmaa Sami Mohammed Mansour ; Supervised Wagih A. Gad Elsaid , Sabry D. Morgan , Mahmoud D. Elhariri 
 - Cairo :   Asmaa Sami Mohammed Mansour ,   2017 
 - 139 P. :   charts , facsimiles ;   25cm 
<br/><br/>Thesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology 
<br/><br/> This study applied on 141 bovine raw milk samples. Each sample was subjected for bacteriological examination. This work explored the high prevalence of S. aureus in raw milk samples. Out of 141 samples of raw milk studied; 24 of them were contaminated by S. aureus, corresponding to 17% of samples contaminated. Molecular identification of S. aureus isolates was completed using PCR revealing that 23 out of 24 isolates contained nuc gene with a percentage 95.8%. The risk of poisoning from ingestion of milk containing enterotoxins from S. aureus is well known so the enterotoxin production by S. aureus isolates was examined. It was evident that of 23 S. aureus isolates; 6 (26.1%) proved to be enterotoxigenic while non-enterotoxigenic isolates constituted 17 (73.9%). Testing of the isolates for the presence of nuc and coa genes, using unmodified gold nanoparticles, reported that all 23 isolates of S. aureus expressed nuc and coa genes 
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<br/><br/><label>Subjects--Index Terms: </label>Enterotoxin genes S. aureus Unmodified gold nanoparticles