Peter Ehab Mouris Eskandar Beshay <br/><br/>
Emulsion-based microfluidics for the label-free identification of rare cells /  
 إكتشاف خلايا نادرة حية بإستخدام تكنولوجيا مستحلبات الموائع الدقيقة 
Peter Ehab Mouris Eskandar Beshay ; Supervised Yasser Hussein Anis 
 - Cairo :   Peter Ehab Mouris Eskandar Beshay ,   2017 
 - 108 P. :   charts , facsimiles ;   30cm 
<br/><br/>Thesis (M.Sc.) - Cairo University - Faculty of Engineering - Department of Mechanical Design and Production 
<br/><br/> This thesis introduces an emulsion-based label-free cell identication technique that utilizes cell dynamics inside an emulsion to recognize its type. First, the single cells are encapsulated in emulsions, generated inside a poly-dimethylsiloxane (PDMS) microuidic c hip. When an emulsion is disturbed from equilibrium, an internal rotational ow eld is generated; causing the encapsulated cell to rotate in an elliptical orbit. High speed cameras are used for monitoring cell rotation. Tracking the cell rotation inside of the emulsion could reveal cell type. Experimentally, two dierent cell lines (malignant MCF-7 and non-malignant MCF-10A breast epithelial cell lines) were tested to study their dynamic behavior using the proposed sheath ow microchannel. Computer vision techniques were used to analyze and track cell rotations. Experiments showed  that the MCF-7 and MCF-10A behaved dierently inside emulsions larger than  145æm in diameter and at ow rates higher than 0.7 æL/min. The nding of this thesis proves the potential capability of emulsion based techniques in  identifying rare cells 
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<br/><br/><label>Subjects--Index Terms: </label>CTCs Label-free isolation Microfluidics