Biochemical and molecular studies of some plants as anticancer /
دراسات بيوكيميائية وجزيئية علي بعض النباتات كمضادات للسرطان
Huda Mohamedahmed Tallima ; Supervised Hany Abdelaziz Elshemy , Faten Mohamed Abouelella , Mahgoub Mohamed Ahmed
- Cairo : Huda Mohamed Ahmed Tallima , 2017
- 124 P. : charts , facsimiles ; 25cm
Thesis (M.Sc.) - Cairo University - Faculty of Agriculture - Department of Agricultural Biochemistry
Medicinal plants play an important role in our life. Therefore, this work aimed to study the antioxidant, anti-inflammatory and anticancer effects of the ethanolic extract of Rosmarinus officinalis, Acacia nolitca, Borage officinalis and Ammi majus. Primary compounds were determined in dried plants. Preliminary phytochemical screening of the ethanolic extracts of the four plants demonstrated the presence or absence of terpens, tannins and flavonoids. Quantitative analysis was conducted to determine total phenols and alkaloids for all extracts. Antioxidant activity was determined by DPPH at various concentrations.The effects to quench the DPPH radical was estimated in dose dependent manner for the four plants and the best results were at 1000 ppm for Rosmarinus officinalis.The anti-inflammatory activity was determined by using carrageenan induced rats paw edema compared to indomethacin against the four plants with doses 5, 10, 20 mg/kg. The highest effect for both Rosmarinus officinalis and Acacia nolitca was at 20mg/kg where Borage officinalis was at 10 mg /kg and Ammi majus was at 5mg/kg. The anticancer activity was determined inA549lung cancer cell line with the ethanolic extract of Rosmarinus officinalis, Acacia nolitca, against doxorubcine as a positive control by using neutral red assay.Different concentrations (12.5, 25, 50, 75, 100 ppm) were used. Rosmarinus officinalis recorded higher anticancer activity (100%) at 100 ppm with no cell viability while Acacia nolitca recorded 28.57%. The expression of apoptosis genes BCl2-Bax was determined.The data showed that BAX expression was increased after treatment by both plants extracts while BCL2 was decreased after treatment by Acacia nolitca and Rosmarinus officinalisextracts with 0.028 and 0.18 fold change respectively.TNF-Alpha and 8-hydroxy-desoxyguanosine (8-OHdG) were determined to investigate the anticancer mechanism of the ethanolic extract for both Rosmarinus officinalis and Acacia nolitca using IC50. Rosmarinus officinalis recorded the higher effect