TY  - BOOK
AU  - Manar Mahmoud Imam Ibrahim
AU  - Azza Mohamed Elamir, 
AU  - Neamat Ame , 
AU  - Noha Ahmed Mahana , 
TI  - Diagnosis of acute and chronic schistosomiasis haematobium using purified 26 kDa schistosoma-specific protein / 
PY  - 2017///
CY  - Cairo : 
PB  - Manar Mahmoud Imam Ibrahim , 
KW  - Diagnosis
KW  - PSPA-26
KW  - S. haematobium
N1  - Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Zoology; Issued also as CD
N2  - One of the world wide major public health problems is the schistosomiasis that is caused by Schistosoma (S.) heamatobium. It is also one of the main concerns for the public health community in Egypt. There are several immunodiagnostic methods used for that diagnosis of such disease, but some are more sensitive and specific than others. The purified 26 kDa Schistosoma-specific protein (PSPA-26) detection in serum samples is found out to be more valuable in diagnosis; it also helps in the early diagnosis which will lead to the early treatment before the irreversible damage takes place. PSPA-26 was purified from whole worms by DEAESephadex G-75 ion exchange chromatography and then was injected into rabbits to produce specific polyclonal antibodies(anti-PSPA-26 pAb)which is then used as a primary capture in the indirect ELISA technique to reveal its reactivity using infected human sera.The anti-PSPA-26 was then labeled with horse-radish peroxidase (HRP) and used as a secondary capture.Sandwich ELISA was done for serum samples of human and hamsters infected with S. heamatobium.The results revealed a sensitivity of 85% for human and 80% for hamster's samples,and a specificity of 95% for human and 91.1% for hamsters samples by comparing them with those infected with other parasites and control samples. Data obtained concluded that PSPA-26 antigen can be used as a diagnostic marker for S. haematobium infection using the sandwich ELISA which is cost effective and applicable technique
ER  -