A Study on the potential in vitro antimycobacterial activity of natural product(s) from selected wild plants /
دراسة علي الفاعلية المعملية المحتملة المضادة للميكوباكتيريام لمنتجات طبيعية المستخرجة من نباتات برية مختارة
Nesreen Ahmed Safwat ; Supervised Mohammed A. Ramadan , Ramy K. Aziz , Khadegah F. Amer
- Cairo : Nesreen Ahmed Safwat , 2018
- 156 P. : charts , facsimiles ; 25cm
Thesis (Ph.D.) - Cairo University - Faculty of Pharmacy - Department of Microbiology and Immunology
Tuberculosis remains a major health problem accentuated by the rise of resistance to all available drugs. Therefore, this study was launched to discover novel antituberculosis agents from natural products.Twelve wild plants were in vitro screened for their activity against typical and atypical Mycobacterium species. The most active plant, Euphorbia paralias, was further fractionated with different organic solvents, and the activity of the obtained fractions was determined by agar diffusion and broth microdilution methods.The methanol fraction was found to be the most active against Mycobacterium spp.,and was non-toxic in doses up to 10 g/kg of animal weight. The main component was separated by column chromatography then identified by ultraviolet spectroscopy and nuclear magnetic resonance analysis as quercetin-3-O-Ý-D-glucoside. Treatment with quercetin-3-O-Ý-D-glucoside inhibited cell survival of HepG2 and A549 cells in a dose and time dependent manner. Molecular docking analysis suggested that quercetin-3-O-Ý-D-glucoside inhibits the glutamine synthetase enzyme, a promising target for the development of drugs for tuberculosis therapy. This current study prediction was confirmed by the in vitro glutamine synthetase biosynthesis assay